paraformaldehyde (Thermo Fisher)

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Thermo Fisher paraformaldehyde
Paraformaldehyde, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/paraformaldehyde+pbs/Paraformaldehyde+Solution%2C+4%25+in+PBS/pmc13284439-129-8-33
Average 99 stars, based on 1 article reviews

paraformaldehyde - by Bioz Stars, 2026-09

99/100 stars

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Labeling:

Article Title: Microenvironmental TGF-β is an early driver of NF1-associated tumor formation.
Article Snippet: The following day, cells were washed 3 times in SATO medium and incubated for 30h in SATO medium without insulin, containing 10 μM EdU (Invitrogen), in the presence or absence of 100ng/mL NRG1 (R&D), 50ng/mL IGF1 (BioScience), and 10ng/mL TGF-β (R&D), alone or in combination. .. Cells were fixed in 4% paraformaldehyde/ PBS for 15 min at RT and labeled for EdU and Hoechst following the manufacturer’s instructions (Invitrogen). ..

Incubation:

Article Title: The phosphate exporter XPR1 regulates a gasdermin D–independent mature IL-1β secretion pathway in LPS-stimulated human monocytic cells
Article Snippet: .. After 6 h of LPS stimulation, cells were washed with PBS, fixed for 15 min in 4% paraformaldehyde/PBS (Invitrogen #FB002), washed with PBS, incubated for 15 min in permeabilization buffer (PBS-0.5% Triton X100, Sigma X-100), and incubated for 30 min in blocking buffer (PBS-1% BSA-1% goat serum-0.1% Triton X-100; BSA, Sigma #A7030; goat serum, Millipore S26). .. Cells were then incubated overnight at 4 °C in the first antibody diluted 1:100 in blocking buffer (anti-XPR1 Sigma Prestige #HPA016557), washed 3 × 5 min in PBS-0.1% Triton and incubated for 1 h at room temperature with an Alexa 488–conjugated secondary antibody (Invitrogen #A48282).

Blocking Assay:

Infection:

Article Title: SAMHD1 depletion restricts SARS-CoV-2 infection by suppressing HNF1-dependent ACE2 expression in lung epithelial cells.
Article Snippet: .. At 72 hpi, infected cells were overnight fixed with 4% paraformaldehyde/PBS (ThermoFisher Scientific, Cat. no. J19943.K2). ..

Article Title: SAMHD1 depletion restricts SARS-CoV-2 infection by suppressing HNF1-dependent ACE2 expression in lung epithelial cells
Article Snippet: .. At 72 hpi, infected cells were overnight fixed with 4% paraformaldehyde/PBS (ThermoFisher Scientific, Cat. no. J19943.K2). ..

Paraffin Wax:

Article Title: Tandem single-cell epigenetic and transcriptomic analysis reveals spatiotemporally distinct genomic features among testis cell lineages
Article Snippet: .. Briefly, testes were collected and fixed in 4% paraformaldehyde/PBS (Thermo Fisher Scientific, 28908) at 4°C for specified time frames depending on the animal age, dehydrated in an ethanol/xylenes series, and embedded in paraffin wax. ..

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